The Basic Principles Of flow cytometer
The Basic Principles Of flow cytometer
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Info-enriched characterization of cell populations. Morphological data from pictures can increase to the richness of flow cytometry information. One example is, the determine reveals an normally standard apoptosis assay employing Annexin V and PI, including mobile imaging to characterize cells in Each individual population to reveal morphologically unique functions. These insights could not happen to be attained from multiplex staining by yourself.
Irrespective of whether you’re a novice or an authority, discover our resources to progress your analysis and knowledge in flow cytometry.
Mix acoustic focusing engineering with substantial-parameter analysis for adaptable spectral unmixing and traditional workflows.
The award-profitable Invitrogen Attune CytPix Flow Cytometer brings together acoustic concentrating by using a significant-pace brightfield digital camera for simultaneous higher throughput flow cytometry and significant resolution brightfield imaging.
"An image just isn't fairly truly worth 1000 dots, however it really helps you to see photographs. It is great to see images that correlate with staining. For instance, AnnexinV-constructive cells which might be morphologically distinct than balanced cells, or CD14+ monocytes which might be larger and more textured than CD3+/CD4+/CD14- lymphocytes.
On top of that, it is illuminating to discover the quantity of doublets slip by means of traditional singlet gates, and fantastic to verify the status of sudden double-constructive gatherings in my Evaluation."
Optimizing gating tactic. Even strong guide singlet gating is error-inclined and continues to be a subjective decision issue in Pretty much all flow cytometry assays. Imaging can be employed to substantiate and alter gates to include only single cells of desire.
Steady image excellent even at substantial throughput Acoustic focusing and also a high-speed camera combine to impression these T cells continually at small or large flow costs. Very easily adjust emphasis and digital camera options to meet experimental specifications.
The Attune NxT cytometer may be the responsible workhorse instrument your lab can rely on for consistent and trusted performance. The Attune CytPix design includes a significant-pace brightfield digital camera and AI-driven impression Evaluation computer software, with current upgrades like machine Studying resources and an intuitive user interface, enabling Improved knowledge precision, accuracy, and more rapidly insights into sample biology.
Buyers can check out occasion photographs in real-time to confirm sample quality, improve protocols, and record the morphology of every mobile population identified for Evaluation. Imaging seize also Advantages from acoustic concentrating, which helps to placement the cells to make sure that a sharp, centered picture is received.
Sound waves empower specific cell alignment, causing Extraordinary knowledge high-quality and ~10X more rapidly sample flow prices.
We Earlier demonstrated the strength of imaging CAR-T/Ramos cell interactions. Let’s check out just the population of finest desire, the double beneficial activities, To find out more. We could now use extended morphological parameters (here, circularity and skewness of intensity) to even further take a look at the capabilities of such populations and refine gating on these events, rising information robustness.
Designed-in dimensionality reduction resources greater resolution into populations. Fluorescently gated populations back-gated onto UMAP plot to substantiate anticipated populace resolution in lysed full human blood. Consumer can check out occasions from Just about every cluster for Visible affirmation of mobile phenotypes.
No wash/no lyse leukocyte Assessment with imaging. Cells had been obtained from 24-hour-aged blood by dilution in 1 mM EDTA ( Qualified UX/UI enables buyers to teach AI models for expanded purposes. (A) The “Coach Design” attribute supplies annotation instruments and genuine-time product education precision.
At the heart of each and every Attune Flow Cytometer lies our groundbreaking acoustic focusing technology, which utilizes sound waves to meticulously align cells inside the flow cell. This innovative method surpasses standard hydrodynamic approaches, leading to a lot quicker processing speeds, Fantastic facts high quality, and elevated sample throughput.
Since the cell line experienced Beforehand been analyzed around the Attune CytPix Flow Cytometer, the scientists went back again to the images and ended up in the position to doc the microbial infection a minimum of five times previously.
Agilent Value Assure assures you a minimum of 10 years of instrument use within the date you purchase. Usually, We are going to credit rating you Using the residual value of the technique toward an upgraded design.
Right here, website we present that by gating techniques applying picture-centered quantitative parameters can distinguish interacting cells from coincident situations to extra accurately review interacting cells.